Browsing by Author "Ilhamjaya Patellongi"
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- ItemAssociation of Vitamin D Receptor Polymorphism (rs2228570, rs1544410, rs7975232, and rs731236) and Macrophage Migration Inhibitory Factor -173 G/C (rs755622) with the Susceptibility of Active Pulmonary Tuberculosis in Makassar, Indonesia(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-10-16) Najdah Hidayah; Irawaty Djaharuddin; Ahyar Ahmad; Rosdiana Natzir; Ilhamjaya Patellongi; Agussalim Bukhari; Irda Handayan; Andi Tenriola; Subair Subair; Handayani Halik; Muhammad Nasrum MassiBACKGROUND: The study of Vitamin D Receptor (VDR) and Macrophage Migration Inhibitory Factor (MIF) polymorphisms associated with active pulmonary tuberculosis (TB) (ATB) presents varying results. AIM: This study aimed to investigate the association between VDR rs2228570, rs1544410, rs7975232, and rs731236 and MIF -173 G/C (rs755622) single-nucleotide polymorphism (SNP), with susceptibility of developing ATB, and positivity of interferon-gamma release assay (IGRA) results (in household contact). MATERIALS AND METHODS: This study involved 83 ATB and 73 household contacts in Makassar. We checked IGRA based on ELISA in household contacts by using QuantiFERON-TB Gold Plus test, and we found that 61.64% (n = 45) of household contacts had positive IGRA. Polymorphism examination was carried out by Sanger sequencing. RESULTS: VDR rs2228570 T/T and T/C-T/T were significantly associated with a higher risk of active TB. VDR rs7975232 G/G genotype was associated with an increased risk of developing active TB compared to T/T-T/G. Haplotype analysis of VDR rs2228570, rs1544410, rs7975232, and rs731236 and combination with MIF rs755622 demonstrated that TGGTG was observed to have a higher risk of TB. CONCLUSIONS: The combination of VDR and MIF variants may contribute to the susceptibility of active tuberculosis disease.
- ItemComposite Bacterial Infection Index and Serum Amyloid A Protein in Pulmonary Tuberculosis Patients and their Household Contacts in Makassar(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-06-10) Irda Handayani; Muhammad Nasrum Massi; Yanti Leman; Rosdiana Natzir; Ilhamjaya Patellongi; Subair Subair; Najdah Hidayah; Ayu Andini Wulandari; Handayani HalikBACKGROUND: Early diagnosis of tuberculosis (TB) cases in limited resource remains challenging. It is urgent to identify the new diagnostic tools which can control the spread of disease with accurate and rapid test. AIM: This study aimed to investigate the levels of infection markers: Composite bacterial infection index (CBII) and serum amyloid A (SAA) protein in pulmonary TB (PTB), and their healthy household contacts, as the alternative diagnostic markers for TB. METHODS: CBII and SAA were measured from 44 new PTB patients, and 31 household contact serum samples. The value of CBII was calculated from neutrophils, lymphocytes, monocytes, erythrocyte sedimentation rate, and high-sensitivity C-reactive protein (hs-CRP) level. hs-CRP and SAA levels were quantified from their serum samples using ELISA. QuantiFERON-TB Gold Plus (interferon gamma release assay [IGRA]) was used to screen latent TB infection among household contacts. RESULTS: Among 31 household contacts, there were 24 positive IGRA results and the rest (n = 7) had negative results. PTB patients exhibited significantly higher level CBII in the serum specimens, than those in household contact (p < 0.0001). There was no significant difference in the SAA level between TB cases and household contacts (p = 0.679). CONCLUSIONS: CBII can be used as one of the biomarkers for the identification of PTB from the serum specimens.