Browsing by Author "Najdah Hidayah"
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- ItemAssociation of Vitamin D Receptor Polymorphism (rs2228570, rs1544410, rs7975232, and rs731236) and Macrophage Migration Inhibitory Factor -173 G/C (rs755622) with the Susceptibility of Active Pulmonary Tuberculosis in Makassar, Indonesia(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-10-16) Najdah Hidayah; Irawaty Djaharuddin; Ahyar Ahmad; Rosdiana Natzir; Ilhamjaya Patellongi; Agussalim Bukhari; Irda Handayan; Andi Tenriola; Subair Subair; Handayani Halik; Muhammad Nasrum MassiBACKGROUND: The study of Vitamin D Receptor (VDR) and Macrophage Migration Inhibitory Factor (MIF) polymorphisms associated with active pulmonary tuberculosis (TB) (ATB) presents varying results. AIM: This study aimed to investigate the association between VDR rs2228570, rs1544410, rs7975232, and rs731236 and MIF -173 G/C (rs755622) single-nucleotide polymorphism (SNP), with susceptibility of developing ATB, and positivity of interferon-gamma release assay (IGRA) results (in household contact). MATERIALS AND METHODS: This study involved 83 ATB and 73 household contacts in Makassar. We checked IGRA based on ELISA in household contacts by using QuantiFERON-TB Gold Plus test, and we found that 61.64% (n = 45) of household contacts had positive IGRA. Polymorphism examination was carried out by Sanger sequencing. RESULTS: VDR rs2228570 T/T and T/C-T/T were significantly associated with a higher risk of active TB. VDR rs7975232 G/G genotype was associated with an increased risk of developing active TB compared to T/T-T/G. Haplotype analysis of VDR rs2228570, rs1544410, rs7975232, and rs731236 and combination with MIF rs755622 demonstrated that TGGTG was observed to have a higher risk of TB. CONCLUSIONS: The combination of VDR and MIF variants may contribute to the susceptibility of active tuberculosis disease.
- ItemComposite Bacterial Infection Index and Serum Amyloid A Protein in Pulmonary Tuberculosis Patients and their Household Contacts in Makassar(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-06-10) Irda Handayani; Muhammad Nasrum Massi; Yanti Leman; Rosdiana Natzir; Ilhamjaya Patellongi; Subair Subair; Najdah Hidayah; Ayu Andini Wulandari; Handayani HalikBACKGROUND: Early diagnosis of tuberculosis (TB) cases in limited resource remains challenging. It is urgent to identify the new diagnostic tools which can control the spread of disease with accurate and rapid test. AIM: This study aimed to investigate the levels of infection markers: Composite bacterial infection index (CBII) and serum amyloid A (SAA) protein in pulmonary TB (PTB), and their healthy household contacts, as the alternative diagnostic markers for TB. METHODS: CBII and SAA were measured from 44 new PTB patients, and 31 household contact serum samples. The value of CBII was calculated from neutrophils, lymphocytes, monocytes, erythrocyte sedimentation rate, and high-sensitivity C-reactive protein (hs-CRP) level. hs-CRP and SAA levels were quantified from their serum samples using ELISA. QuantiFERON-TB Gold Plus (interferon gamma release assay [IGRA]) was used to screen latent TB infection among household contacts. RESULTS: Among 31 household contacts, there were 24 positive IGRA results and the rest (n = 7) had negative results. PTB patients exhibited significantly higher level CBII in the serum specimens, than those in household contact (p < 0.0001). There was no significant difference in the SAA level between TB cases and household contacts (p = 0.679). CONCLUSIONS: CBII can be used as one of the biomarkers for the identification of PTB from the serum specimens.
- ItemmicroRNA hsa-miR-425-5p and hsa-miR-4523 expressions as biomarkers of active pulmonary tuberculosis, latent tuberculosis infection, and lymph node tuberculosis(KeAi Communications Co., 2023-07-14) Muhammad Nasrum Massi; Najdah Hidayah; Irda Handayani; Israini Wiyulanda Iskandar; Fathul Djannah; Nirmawati Angria; Handayani HalikStudies on miRNA highlight its significance as an immunomarker for several diseases, including tuberculosis. This study aimed to determine the difference between miR-425-5p and miR-4523 expressions in patients with active pulmonary TB (PTB), latent TB infection (LTBI), and lymph node TB (LNTB), whose diagnosis remains challenging. Methods: This case–control study was performed on blood samples obtained from 23 patients with PTB, 21 with LTBI, 21 with LNTB, and 25 healthy controls (HC). miRNA hsa-miR-425-5p and hsa-miR-4523 expression levels were measured by RT-qPCR. Statistical analyses were performed using SPSS version 25.0. Results: RT-qPCR showed that hsa-mir-425-5p and hsa-mir-4523 expression levels were significantly different among the four groups (PTB, LTBI, LNTB, and HCs). The hsa-mir-425-5p miRNA expression level in LNTB was higher than that in LTBI (p = 0.003). Meanwhile, the hsa-mir-4523 miRNA expression was downregulated in PTB and LNTB than in LTBI (p < 0.0001 and p = 0.015, respectively). The ROC analysis of a single sample showed that only mir-4523 could discriminate LTBI and HCs, with an AUC of 0.829 (p < 0.001). The ROC curve of each miRNA was further analyzed after logistic regression by adjusting for sex and age. The combination of both miRNAs was also analyzed. The model that analyzed the combination of both miRNAs after adjusting for age had the best performance in differentiating LNTB from LTBI, with an AUC of 0.97 (p < 0.001). Conclusion: miRNA hsa-mir-425-5p was upregulated and miRNA hsa-mir-4523 was downregulated in PTB and LNTB than in LTBI.
- ItemThe Significance of Differences in Melanocortin 3 Levels and their Relationship with Pulmonary Tuberculosis and Body Mass Index(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-08-21) Andi Tenriola; Najdah Hidayah; Subair Subair; Muhammad Nasrum Massi; Irda Handayani; Rosdiana Natzir; Irawaty Djaharuddin; Handayani HalikBACKGROUND: Melanocortin 3 Receptors (MC3R) levels plays a role in many biological systems, including energy homeostasis and regulation of fat metabolism. However, very few have researched the relationship between MC3R and tuberculosis (TB) and body mass index. AIM: This study explores the differences in serum MC3R levels in active TB, household contacts, and control groups, as well as at different body mass index status. This study tries to find out the relationship between MC3R and other variables. METHODS AND MATERIALS: Blood samples were taken from 53 active TB patients, 49 household contacts, and 30 healthy people as controls. The 132 samples were subjected to IGRA and ELISA examinations to determine differences in MC3R levels in all groups. RESULTS: The highest mean of MC3R levels were found in the active TB group at 1.259.55 (p = 0.028) and had a positive correlation with a value of p = 0.008. In the sex group, men had the highest levels (p = 0.551). In the 30–49 year age group, the median value increased significantly in the three groups (p = 0.028), and there was a correlation between MC3R and the 17–29 year age group, although the correlation was negative (p = 0.021), in the 30–49 year age group with a positive correlation (p = 0.050). The mean MC3R value increased significantly in the overweight group in the three groups (p = 0.006) but did not significantly correlate. CONCLUSION: The high level of MC3R in TB patients is related to its role as a defence against microbes that enter the body through the immune process to prevent further infection and inflammation. Meanwhile, high levels of MC3R in excess Body mass index were associated with the function of MC3R as an inhibitor of pro-opiomelanocortin (POMC) neurons to release α-MSH.