FIRA WULANDARI SUDIRMAN2025-08-042025-08-04https://repository.unimerz.ac.id/handle/123456789/539The white turmeric rhizome plant (Curcuma zedoaria Rosc.) is a plant that contains secondary metabolites, one of which is flavonoids which can be used as antibacterial substances. So the aim of this research is to determine whether the ethyl acetate fraction of white turmeric rhizomes (Curcuma zedoaria Rosc.) can be formulated as a hand sanitizer gel preparation, and has antibacterial activity and to find out what the Minimum Inhibitory Concentration (MIC) is for Staphylococcus aureus and Escherichia coli bacteria. This research method is laboratory experimental research, using the maceration method with the disc diffusion test method. At concentrations of F1 (5%), F2 (7%) and F3 (9%), K(-) without fraction and K+ (ANTIS). The results of the research evaluation at concentrations of F1 (5%), F2 (7%) and F3 (9%) meet the physical and chemical requirements. Antibacterial activity test against Staphylococcus aureus bacteria at F1 (5%) inhibition zone 11.6 mm (strong), F2 (7%) inhibition zone 12.4 mm (strong) and F3 (9%) inhibition zone 13.9 mm ( strong) and K+ inhibition zone 16.9 mm and in Escherichia coli bacteria F1 (5%) inhibition zone 10.7 mm (strong), F2 (7%) inhibition zone 11.5 mm (strong) and F3 (9%) inhibition zone 12.6 mm (strong) and K+ inhibition zone 16.4 mm. It was concluded that the minimum inhibitory concentration (MIC), namely 5% in F1, could inhibit bacterial growth in the strong category.Antibacterial activityHand sanitizerethyl Acetate raction of white turmeric rhizome (Curcuma zedoaria Rosc.)Staphylococcus Aureus and Escherichia Coli.Thesis