Browsing by Author "Andi Sulfyana Sumang"
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- ItemPROFILING THE TOTAL NUMBER OF BACTERIA IN THE DIGESTIVE TRACT OF CHILDREN WITH STUNTING CONDITIONS(Universitas Islam Negeri Alauddin Makassar, 2022-12-29) Hasnawati S; Syamsyuryana Sabar; Andi Sulfyana SumangStunting is a failure to thrive in children under five caused by various factors, including lack of nutritional intake, experiencing repeated infections, and inadequate psychosocial stimulation. The excess of bacteria, especially pathogenic bacteria in the gastrointestinal tract, causes inflammation, gut microbiome imbalance, and malabsorption of nutrients; this impacts growth disturbance resulting in stunting. Stunting can occur in the first 1000 days of birth and is related to many factors, including socioeconomic status, food intake, infection, mother’s nutritional status, infectious diseases, micronutrient deficiencies, and the environment. Stunting does not only have an impact on individuals who experience it but also on the wheels of the economy and national development. This is because human resources with stunting are of lower quality than human resources without stunting. Using the qPCR method, this study aims to determine the total number of bacteria in the digestive tract of checking toddlers in Bone-bone Village and Pepandungan Village, Baraka District, Enrekang Regency. This study used a molecular method, namely the Quantitative PCR (Q-PCR) method with 16SrRNA primers to detect total bacteria. The subjects of this study were stunted toddlers in Bone-Bone Village and Pepandungan Village, totaling 21 people plus ten controls. This type of research is a quantitative descriptive study using a cross-sectional study design to identify the total number of bacteria present in the feces of stunted toddlers. The results obtained from the q-PCR method showed that the average total number of small children's bacteria was less, namely 2.28 log DNA Copies/gram compared to normal children of 5.95 log DNA Copies/gram with a difference between the two subject groups of 3 .67 log DNA Copies/gram. The results obtained show that bacteria do not cause the incidence of stunting in the two villages.