Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
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Date
2020-01-22
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Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia
Abstract
Alvionita DN, Rahayu S, Mubarik NR. 2020. Characterization, identification, and analysis bioactive compound of endophytic bacteria from Hoya multiflora Blume. Biodiversitas 21: 195-202. Hoya multiflora Blume has been used for various purposes. Extract hoya has been used antibacterial, stomachache, skin diseases and natural insecticidal. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is by using endophytic bacteria. The purposes of this research were to isolate, characterize the endophytic bacteria from H. multiflora, identify its bioactive compound and to test its antimicrobial activity against two pathogenic bacteria. A total of 18 isolates from H. multiflora Blume were successfully obtained. Based on antimicrobial test, isolate HMA 2 was able to inhibit Escherichia coli, isolate HMD 6 inhibited Staphylococcus aureus and two isolates inhibited both of pathogenic bacteria (i.e isolate HMB 1 and HMD 4). In addition, minimum inhibitory concentration (MIC) of ethyl acetate extract from HMB 1 was 0.625 mg/mL, while from HMD 4 was 0.125 mg/mL, both against E. coli and S. aureus. Based on 16S rRNA gene sequencing analysis, HMB 1 was the most similar (99.91%) with Bacillus siamensis KCTC-13613 and HMD 4 was the most similar (100%) with Bacillus aryabhattaiB82W22. Using GC-MS and compared with database WILLEY9THN08, isolate HMB 1 identified compounds such as 3-benzyl-1,4-diaza-2,5-dioxobicyclo and 13 Docosenamide, isolate HMD 4 contained compounds such as linolenic acid, 1,2-benzene dicarboxylic acid, oleic acid amide, 1-nonadecene, and stigmasterol. More than, there are 3 compound identified from isolates HMB 1 and HMD 4: phenol, 2,4-bis (1,1-dimethyl); neophytadiene dan pyrollo [1,2-a]pyrazine-1,4-dione,hexahydro-3- (phenylmethyl), respectively
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Alvionita, D. N., Rahayu, S. R. I., & MUBARIK, N. R. (2020). Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume. Biodiversitas Journal of Biological Diversity, 21(1).