ANALISIS KADAR ASAM RETINOAT DALAM SEDIAAN KRIM PEMUTIH YANG BEREDAR DI KOTA MAKASSAR DENGAN METODE SPEKTROFOTOMETRI UV-Vis

Abstract

Cosmetics is product that is designed to be used on parts of the human body and is not intended to treat a particular disease, but only as a cleanser, beautify, increase attractiveness or change appearance. Retinoic acid is the derivative of vitamin A in the form of tretinoin (all-trans retinoic acid) which is indicated for use in the treatment of acne. Retinoic acid is also an ingredient that is prohibited from being used in cosmetic preparations because it can cause dry skin, burning and teratogenicity (defects in the fetus). This research aimed to determine wheter the preparation of whitening cream circulating in the city of Makassar contains retinoic acid and to determine the retinoic acid levels contained in the preparation of the whitening cream. Qualitative analysis of retinoic acid was carrier out by using the Thin Layer Chromatography (TLC) method which produces dark spots when observed under a nm lamp and by UV-Vis spectrophotometry method which will produce a maximum wavelength equal to or almost the same as the comparison standard, namely 344,10 nm. The assay was carried out by means of UV-Vis spectrophotometry at the wafelegth of 344,10 nm. The results showed that there were 3 positive samples containing retinoic acid, namely samples A, B, and C. The retinoic acid content in sample A was 0,025%, in sample B was 0,014%, and in sample C was 0,036%.