PENENTUAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG SINGKONG (ManihotesculentaCrantz) DENGAN METODE 1,1-Diphenyl-2-picrylhydrazyl (DPPH

Abstract

Cassava bark is a part of the cassava plant which is still underutilized besides being used as animal feed and wasted as waste. Each cassava can usually produce 10-15% cassava bark. The amount of waste produced can cause accumulation which results in environmental damage if not utilized properly (Asiah, 2019). This research aims to determine the antioxidant activity and Crantz using the DPPH (1,1-diphenyl-2-pikrihidrazil) method which was measured using a UV-Vis spectrophotometer. Extraction used maceration method of 96% ethanol as solvent. Determination of antioxidants ethanol extract of cassava bark (Manihot esculenta Crantz) by DPPH method and using vitamin C was used as a reference. Vitamin C was made in four series of concentrations, namely 2, 4, 6 and 8 ppm while the ethanol extract of cassava bark, was 20, 40, 60 and 80 ppm. From several concentration series, 2 ml was taken and 1 ml of 50 ppm DPPH was added, then the mixture obtained was incubated for 30 minutes in a place protected from light. The absorbance was measured at a wavelength of 515 nm. The results showed that the ethanol extract of cassava bark has a very strong antioxidant activity based on the IC50 value obtained which is 3.847 mg/L. Therefore, the ethanol extract of cassave bark can be developed as a natural antioxidant