Association of Vitamin D Receptor Polymorphism (rs2228570, rs1544410, rs7975232, and rs731236) and Macrophage Migration Inhibitory Factor -173 G/C (rs755622) with the Susceptibility of Active Pulmonary Tuberculosis in Makassar, Indonesia
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Date
2021-10-16
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Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia
Abstract
BACKGROUND: The study of Vitamin D Receptor (VDR) and Macrophage Migration Inhibitory Factor (MIF) polymorphisms associated with active pulmonary tuberculosis (TB) (ATB) presents varying results.
AIM: This study aimed to investigate the association between VDR rs2228570, rs1544410, rs7975232, and rs731236 and MIF -173 G/C (rs755622) single-nucleotide polymorphism (SNP), with susceptibility of developing ATB, and positivity of interferon-gamma release assay (IGRA) results (in household contact).
MATERIALS AND METHODS: This study involved 83 ATB and 73 household contacts in Makassar. We checked IGRA based on ELISA in household contacts by using QuantiFERON-TB Gold Plus test, and we found that 61.64% (n = 45) of household contacts had positive IGRA. Polymorphism examination was carried out by Sanger sequencing.
RESULTS: VDR rs2228570 T/T and T/C-T/T were significantly associated with a higher risk of active TB. VDR rs7975232 G/G genotype was associated with an increased risk of developing active TB compared to T/T-T/G. Haplotype analysis of VDR rs2228570, rs1544410, rs7975232, and rs731236 and combination with MIF rs755622 demonstrated that TGGTG was observed to have a higher risk of TB.
CONCLUSIONS: The combination of VDR and MIF variants may contribute to the susceptibility of active tuberculosis disease.
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Hidayah, N., Djaharuddin, I., Ahmad, A., Natzir, R., Patellongi, I., Bukhari, A., ... & Massi, M. N. (2021). Association of Vitamin D Receptor Polymorphism (rs2228570, rs1544410, rs7975232, and rs731236) and Macrophage Migration Inhibitory Factor-173 G/C (rs755622) with the Susceptibility of Active Pulmonary Tuberculosis in Makassar, Indonesia. Open Access Macedonian Journal of Medical Sciences, 9(A), 838-848.