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- ItemPembuatan Nano Partikel Kalsium (Ca) dari Limbah Tulang Ikan Patin (Pangasius sp) Menggunakan Metode Ultrasound-Asissted Solvent Extraction(Department of Chemistry, Faculty of Science and Technology UIN Alauddin Makassar., 2020-01-09) Nuramaniyah Taufiq; Risky Nurul Fadlila RNManagement of fish bones asa a source of calcium must be converted into a smaller form so that it is easily absorbed by the body into nanocalsium. In this case, fish bones are seen as giving a lot of advantages because fish are easier to get. The purpose of this study was to utilize catfish (Pangasius Sp) bones as the main ingredient for the manufacture of nanocalcium and extract fish bone lead using the Ultrasonic-Assisted Solvent Extraction method. This research method is characterization. of cat fish bone waste making nano particles using the Ultrasonic-Assisted solvent method, and characterized SEM-EDX. The result showed that the characteristic of catfish bone powder waste were 10,35 % water content, 44,29 % ash content, 1,849 fiber content, 11,47 % fat content, 0,27% protein content, and carbohydrates 33,62%, and SEM result for the morphological size 5.000x magnification, the particle diameter 311,08 nm and EDX was 6 % purity of calcium (Ca).
- ItemCharacterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume(Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, 2020-01-22) DEWINTA NUR ALVIONITA; SRI RAHAYU; NISA RACHMANIA MUBARIKAlvionita DN, Rahayu S, Mubarik NR. 2020. Characterization, identification, and analysis bioactive compound of endophytic bacteria from Hoya multiflora Blume. Biodiversitas 21: 195-202. Hoya multiflora Blume has been used for various purposes. Extract hoya has been used antibacterial, stomachache, skin diseases and natural insecticidal. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is by using endophytic bacteria. The purposes of this research were to isolate, characterize the endophytic bacteria from H. multiflora, identify its bioactive compound and to test its antimicrobial activity against two pathogenic bacteria. A total of 18 isolates from H. multiflora Blume were successfully obtained. Based on antimicrobial test, isolate HMA 2 was able to inhibit Escherichia coli, isolate HMD 6 inhibited Staphylococcus aureus and two isolates inhibited both of pathogenic bacteria (i.e isolate HMB 1 and HMD 4). In addition, minimum inhibitory concentration (MIC) of ethyl acetate extract from HMB 1 was 0.625 mg/mL, while from HMD 4 was 0.125 mg/mL, both against E. coli and S. aureus. Based on 16S rRNA gene sequencing analysis, HMB 1 was the most similar (99.91%) with Bacillus siamensis KCTC-13613 and HMD 4 was the most similar (100%) with Bacillus aryabhattaiB82W22. Using GC-MS and compared with database WILLEY9THN08, isolate HMB 1 identified compounds such as 3-benzyl-1,4-diaza-2,5-dioxobicyclo and 13 Docosenamide, isolate HMD 4 contained compounds such as linolenic acid, 1,2-benzene dicarboxylic acid, oleic acid amide, 1-nonadecene, and stigmasterol. More than, there are 3 compound identified from isolates HMB 1 and HMD 4: phenol, 2,4-bis (1,1-dimethyl); neophytadiene dan pyrollo [1,2-a]pyrazine-1,4-dione,hexahydro-3- (phenylmethyl), respectively
- ItemPEMANFAATAN EKSTRAK DAUN SELEDRI (Apium Graveolens) SEBAGAI ANTIKOAGULAN PENGGANTI EDTA (Ethylene Diamine Tetraacetic Acid) PADA PEMERIKSAAN JUMLAH TROMBOSIT(Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Politeknik Medica Farma Husada Mataram, 2020-10-19) Ika Nurfajri Mentari; Desyani Ariza; Idham HalidCelery (Apium Graveolens) has the same content as EDTA, namely flavonoids which can be used as anticoagulants, these flavonoids play a role in inhibiting the platelet aggregation process so that researchers want to do a research about of celery leaf extract (Apium Graveolens) as a substitute for EDTA (Ethylene Diamine Tetraacetic Acid) anticoagulants. Acid) on the examination of the platelet count. This research method is a laboratory experiment, namely the manufacture of celery leaf extract (Apium Graveolens) and homogenized with blood, which is then examined the number of platelets with 4 treatments. The examination of the platelet count also used the anticoagulant EDTA as a comparison. The results of examining the number of platelets using celery leaf extract with a concentration of 50% and using EDTA anticoagulants have the same results, so it can be stated that celery leaf extract can be used as an alternative anticoagulant as a substitute for EDTA (Ethylene Diamine Tetraacetic Acid) anticoagulant.
- ItemSynthesis and interaction of adenosine-5'-triphosphate with rare earth metal Europium (Eu3+)(American Institute of Physics, 2020-11-06) Santi; Abdul Wahid Wahab; Indah Raya; Ahyar AhmadLanthanides and nucleotides can from attractive organic coordiantion polymers. Our study explore about the reaction of adenosine-5- triphoshate (ATP) with europium and study its bonding characteristic through the FT_IR Spectrum and UV-Vis spectoscopy. The Result Showed that the Eu (III) complex with adenosine-5-triphosphate (ATP) was successfully synthesized and could interact in oxygen atoms from the phospate group and nitrogen atoms from the ademine base with the highest absorption at the ATP.
- ItemThe Effectiveness of Active Fraction of Soursop Leaves Extract (Annona muricata L.) in Total Cholesterol levels(STIFA Makassar, 2021-01-04) Akbar Awaluddin; A Zulkifli A S; Aswar Hasan; Nurzadrina Wahyuddin; AstutiDaun sirsak dilaporkan mempunyai beberapa kandungan yang bermanfaat sebagai obat seperti flavonoid, alkaloid, asam lemak dan daun sirsak dilaporkan memiliki banyak efek farmakologi, salah satunya untuk pengobatan kadar kolesterol darah. Penelitian ini bertujuan mengetahui perbandingan efektivitas fraksi aktif ekstrak etanol daun sirsak (Annona muricata L.) terhadap penurunan kadar kolesterol total mencit jantan (Mus musculus). Metode yang digunakan yaitu menggunakan 5 kelompok mencit jantan yang diberi 5 perlakuan yang berbeda–beda. Kemudian dilanjutkan dengan uji statistik menggunakan analisis LSD. Hasil yang diperoleh menunjukkan bahwa efek penurunan kolesterol total fraksi ekstrak daun sirsak dengan dosis 100mg/kg BB memiliki efek tidak signifikan dengan kontrol positif dengan nilai p=0,501 > 0,05 dan fraksi ekstrak daun sirsak dosis 150 mg/kg BB dengan nilai p=0,580 > 0,05. Sehingga keduanya diduga mampu menurunkan kadar kolesterol total yang hampir sama dengan kontrol positif.
- ItemDETEKSI DAYA TULAR DARAH TALI PUSAT KEPADA BAYI DENGAN IBU HAMIL POSITIF HEPATITIS B(Poltekkes Kemenkes Pontianak, 2021-01-21) Arlitha Deka Yana; Amirah AznawiLatar Belakang: Hepatitis B merupakan penyakit menular yang serius dan umumnya menginfeksi hati yang disebabkan oleh Virus Hepatitis B (HBV) yang dapat menyebabkan penyakit akut maupun kronis. Transmisi Virus Hepatitis (HBV) umumnya pada saat persalinan atau segera setelah persalinan. Tujuan: Penelitian ini bertujuan untuk mendeteksi daya tular darah tali pusat ibu positif HbsAg guna mengetahui profil penularan virus hepatitis B secara vertikal dari Ibu HbsAg positif kepada Bayi. Metode: Jenis Penelitian ini adalah cross Sectional study penelitian dilakukan di Lakukan di Laboratorium Hasanuddin University Medical Research Center (HUM-RC) dengan menggunakan metode ELISA sampel penelitian adalah sampel dari darah ibu dan darah tali pusat bayi dengan ibu positif HbsAg. Dilakukan Analisis terhadap 7 sampel darah ibu dan darah Tali pusat bayi. Hasil: Penelitian menunjukkan adanya 4 kasus darah tali pusat bayi yang positif HbsAg dan 3 kasus Negatif sedangkan pada HbeAg tidak ditemukan di dalam darah tali pusat bayi sedangkan pada ibu didapatka 7 kasus positf HbsAg dan 6 kasus Negatif HbeAg dan 1 kasus Positif HbeAg. Kesimpulan: Sampel darah tali pusat memiliki daya tular yang lemah untuk menularkan Virus HBV Kepada bayi dengan ibu positif HbsAg.
- ItemPolimorfisme Gen Glutamylcysteine Ligase Catalytic (GCLC) Sebagai Deteksi Kerentanan Pasien Tuberkulosis Terhadap Stres Oksidatif Akibat Infeksi Mycobacterium tuberculosis(jurusan biologi FMIPA universitas hassanudin, 2021-03-01) Andi Zulkifli AS; Muh. Nasrum Massi; Rosana AgusThe important genetic factor about susceptibility of oxidative stress in Pulmonary Tuberculosis (PTB) has not been determined. The aim of this study was to analyse the association between polymorphism glutamatecysteine ligase catalytic subunit (GCLC) genes with susceptibility of oxidative stress in PTB in Makassar population of Indonesian. The Methods use is a case-control study was performed on 25 paired subjects with or without PTB, These subjects were selected from result of BTA stain from sputum subjects. The following polymorphisms were genotyped by PCR-RFLP of −129C/T in the GCLC gene. Genotype frequencies and allelic frequencies were analysed. The Results was found There were significant differences in the distribution of genotype frequencies for polymorphism −129C/T in GCLC gene between PTB and non-PTB subjects. The distribution of the allelic frequencies of these genes also showed significant difference between the two groups. Conclusions is The genetic polymorphisms in GCLC −129C/T are associated with susceptibility of Oxidative stress on PTP subject.
- ItemUji Daya Hambat Daun Ketepeng Cina terhadap Pertumbuhan Malassezia furfur Penyebab Tinea versicolor(Forum Dosen Indonesia DPD Sulawesi Barat, 2021-06-01) Ayusti Dirga; Indas Wari Rahman; Muh. YunasdyTinea versicolor merupakan infeksi jamur yang terdapat pada permukaan kulit, penyakit ini disebabkan oleh jamur Malasezzia furfur. Penyakit merupakan infeksi jamur superfisial yang ditandai dengan munculnya makula di permukaan kulit, skuama halus, dan disertai rasa gatal pada kulit. Tujuan penelitian ini untuk mengetahui zona hambat dan menentukan konsentrasi KHM ekstrak daun ketepeng cina (Cassia alata. L) dalam menghambat pertumbuhan jamur Malassezia furfur. Penelitian ini termasuk penelitian eksprimen bersifat deskriftif .Metode yang digunakan yaitu metode Kirby Bauer. Kemudian data yang dianalisis dan disimpulkan. Hasil penelitian ini menunjukkan ekstrak daun ketepeng cina (Cassia alata. L) pada kosentrasi 20%, 40%, dan 60% menunjukkan adanya zona hambat didapatkan nilai rata-rata zona hambat kosentrasi 4.6 mm, 8,2 mm 10,23 mm. Kesimpulan dari 3 kosentasi 20%, 40%, dan 60% dengan nilai rata-rata zona hambat 4.6 mm, 8,2 mm, 10,23 mm dan kontrol positif 13,9 mm sedangkan zona hambat KHM adalah 20%
- ItemUji Sensitivitas Antibiotik terhadap Pertumbuhan Klebseilla Pneumonia dari Sputum Penderita Infeksi Saluran Pernapasan Bawah(Forum Dosen Indonesia DPD Sulawesi Barat, 2021-06-01) Indas Wari Rahman; Arum PrihartiniPneumonia adalah infeksi akut yang mengenai jaringan paru-paru (alveoli) yang dapat disebabkan oleh berbagai mikroorganisme seperti bakteri, virus, dan jamur. Salah satu penyebab utama pada pneumonia komunitas adalah bakteri Klebsiella pneumoniae. Bakteri ini banyak ditemukan di mulut, kulit, dan saluran usus. Sebagian bakteri golongan Klebsiella menjadi sangat resisten terhadap beberapa antibiotik. Penelitian ini bertujuan untuk mengetahui sensitivitas antibiotik penisilin g, ampisilin, amoksisilin, eritromisin, gentamisin, tetrasiklin, sefotaksim, levofloksasin, fosfomisin dan kloramfenikol terhadap pertumbuhan Klebsiella pneumonia. Penelitian ini merupakan penelitian deskriptif analitik dengan metode uji difusi Kirby-Bauer menggunakan sepuluh macam disk antibiotik yang akan diukur zona hambatnya. Bakteri uji yang digunakan diisolasi dari sampel sputum penderita infeksi saluran pernapasan bawah dan dikonfirmasi sebagai spesies Kleibseilla pneumonia dengan uji Vitek 2 Compact. Dari hasil penelitian ini diperoleh bahwa antibiotik jenis Penisilin g, Ampisilin, Amoksisilin, Eritromisin, Sefotaksim dan Levofloksasin memperlihatkan zona hambat Resisten terhadap pertumbuhan Klebsiella pneumoniae, dan antibiotik Gentamisin Intermediet terhadap Klebsiella pneumoniae, sedangkan antibiotik Tetrasiklin, Fosfomisin dan Kloramfenikol Sensitif terhadap Klebsiella pneumoniae sehingga masih baik digunakan untuk menghambat pertumbuhan Klebsiella pneumoniae.
- ItemComposite Bacterial Infection Index and Serum Amyloid A Protein in Pulmonary Tuberculosis Patients and their Household Contacts in Makassar(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-06-10) Irda Handayani; Muhammad Nasrum Massi; Yanti Leman; Rosdiana Natzir; Ilhamjaya Patellongi; Subair Subair; Najdah Hidayah; Ayu Andini Wulandari; Handayani HalikBACKGROUND: Early diagnosis of tuberculosis (TB) cases in limited resource remains challenging. It is urgent to identify the new diagnostic tools which can control the spread of disease with accurate and rapid test. AIM: This study aimed to investigate the levels of infection markers: Composite bacterial infection index (CBII) and serum amyloid A (SAA) protein in pulmonary TB (PTB), and their healthy household contacts, as the alternative diagnostic markers for TB. METHODS: CBII and SAA were measured from 44 new PTB patients, and 31 household contact serum samples. The value of CBII was calculated from neutrophils, lymphocytes, monocytes, erythrocyte sedimentation rate, and high-sensitivity C-reactive protein (hs-CRP) level. hs-CRP and SAA levels were quantified from their serum samples using ELISA. QuantiFERON-TB Gold Plus (interferon gamma release assay [IGRA]) was used to screen latent TB infection among household contacts. RESULTS: Among 31 household contacts, there were 24 positive IGRA results and the rest (n = 7) had negative results. PTB patients exhibited significantly higher level CBII in the serum specimens, than those in household contact (p < 0.0001). There was no significant difference in the SAA level between TB cases and household contacts (p = 0.679). CONCLUSIONS: CBII can be used as one of the biomarkers for the identification of PTB from the serum specimens.
- ItemPerbandingan Tekanan Darah Pada Wanita Obesitas Dan Wanita IMT Normal Sebagai Faktor Resiko Hipertensi Derajat 1(Aliansi Dosen Perguruan Tinggi Swasta Indonesia (ADPERTISI), 2021-07-30) Hartati; Firdayanti Allolinggi; Hijral aswadHipertensi merupakan suatu gangguan yang terjadi pada pembuluh darah yang mengakibatkan suplai oksigen dan nutrisi yang dibawa oleh darah terhambat sampai ke jaringan tubuh dan dapat meningkatkan terjadinya resiko terjadinya penyakit kardiovaskular, Obesitas diketahui menjadi salah satu faktor resiko terjadinya hipertensi. Tujuan dalam penelitian ini adalah untuk mengetahui perbandingan tekanan darah pada wanita obesitas dan wanita imt normal sebagai faktor resiko hipertensi derajat 1. Penelitian ini merupakan penelitian kuantitatif yang melibatkan 104 wanita, yang terdiri 52 wanita obesitas dan 52 wanita memiliki IMT normal dengan usia 39.8 ± 12.6 tahun. Hasil penelitian ini berdasarkan klasifikasi tekanan darah menurut JNC7 menunjukkan pada wanita IMT normal 16 sampel (30.77%) menderita hipertensi derajat 1, sedangkan pada wanita obesitas sebanyak 25 sapmpel (48.08%) menderita hipertensi derajat 1. Berdasarkan uji statistika terdapat perbedaan bermakna pada nilai signifikan Tekanan darah sistolik Asymp sing (2-sided) ρ = 0.00 <α = 0.05. Sedangkan nilai signifikan tekanan darah diastolik tidak menunjukkan peredaan yang bermakna dimana nilai Asymp sing (2-sided) ρ = 0.66 > α = 0.05. Pada penelitian ini disimpulkan bahwa wanita obesitas lebih beresiko menderita hipertensi derajat 1 dibandingkan wanita yang memiliki IMT normal.
- ItemSynthesis, spectroscopic (FT-IR, UV–visible) study, and HOMO-LUMO analysis of adenosine triphosphate (ATP) doped trivalent terbium(Elsevier, 2021-08-05) Santi Santi; Abdul Wahid Wahab; Indah Raya; Ahyar Ahmad; Maming MamingThe lanthanides and nucleotides can form attractive organic coordination polymers. The ligand complex adenosine-5 -triphosphate (ATP) with metal Terbium (Tb 3 + ) has been successfully synthesized. The com- plexes were characterized by FT-IR spectroscopy and UV–vis spectroscopy. The results showed that the metal Tb(III) coordinates with adenosine-5 -triphosphate (ATP) through the electrons in the oxygen atom from the phosphate group and the electrons on the nitrogen atom in the adenine ring. FT-IR proved the existence of ATP-Tb with a vibration band of 908 cm −1 and 349 cm −1 to form a P-O-Tb bond and a vi- bration band of 1635 cm −1 showing the coordination of Nitrogen N1 and N7 atoms as electron donors in terbium metal. The absorption bands at λ= 318.5 nm and 485.5 nm represent the electronic transi- tions of 7 F 6 → 5 H 7 and 7 F 5 → 5 D 4 and the redshift occurs after the addition of Tb 3 + to ATP with the mole ratio of ATP: Tb [1: 2]. The direct band gap obtained from 4.19 to 4.7 eV as a wide band gap semi- conductor material and the indirect band gap obtained from the 5.5–6.0 eV range is an insulator. The HOMO-LUMO energy band gap of the ATP-Tb complex is 3.721 eV. This value is similar to that obtained from experiments using the UV–visible spectrum. Calculations of band gap energies, interactions, and spectral properties of complex compounds can provide the basis for the design of wide band gap semi- conductor materials for rare earth complexes with organic ligands. The electronic properties of ATP-Tb have been calculated Ionization potential (I), electron affinity (A), chemical hardness ( η), chemical soft- ness ( ζ), chemical potential ( μ), electronegativity ( Х ), dan electrophilicity index ( ω).
- ItemPENGARUH PENYIMPANAN SAMPEL PADA PEMERIKSAAN GLUKOSA DARAH DI RSUD TELUK BINTUNI PAPUA BARAT(LPPM Akademi Kesehatan Jhon Paul II Pekanbaru, 2021-08-05) Arlitha Deka Yana; Irna YulianaSalah satu pemeriksaan laboratorium klinik adalah pemeriksaan glukosa darah. Banyaknya Permintaan pemeriksaan kadar glukosa darah sering kali menjadi alasan dilakukan penundaan pemeriksaan di Laboratorium. Tujuan penelitian ini untuk mengetahui Pengaruh penundaan waktu pemeriksaan sampel pada pemeriksaan glukosa darah. Penelitian ini menggunakan jenis penelitian eksperimental dengan melakukan intervensi waktu pada setiap sampel. Intervensi dalam penelitian ini menggunakan lama pendiaman sampel dan terdiri dari pemeriksaan segera, pemeriksaan yang ditunda selama 30 menit, 45 menit dan 60 menit. Hasil penelitian diperoleh bahwa nilai signifikansi 0,000 (p < 0,05), artinya terjadi pengaruh penyimpanan sampel darah yang bermakna pada pemeriksaan glukosa yang didiamkan selama 30 menit,45 menit dan 60 menit. Kesimpulan: Terjadi pengaruh penyimpanan sampel darah pada pemeriksaan glukosa sehingga disarankan untuk melakukan pemeriksaan glukosa darah segera tanpa penundaan.
- ItemThe Significance of Differences in Melanocortin 3 Levels and their Relationship with Pulmonary Tuberculosis and Body Mass Index(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-08-21) Andi Tenriola; Najdah Hidayah; Subair Subair; Muhammad Nasrum Massi; Irda Handayani; Rosdiana Natzir; Irawaty Djaharuddin; Handayani HalikBACKGROUND: Melanocortin 3 Receptors (MC3R) levels plays a role in many biological systems, including energy homeostasis and regulation of fat metabolism. However, very few have researched the relationship between MC3R and tuberculosis (TB) and body mass index. AIM: This study explores the differences in serum MC3R levels in active TB, household contacts, and control groups, as well as at different body mass index status. This study tries to find out the relationship between MC3R and other variables. METHODS AND MATERIALS: Blood samples were taken from 53 active TB patients, 49 household contacts, and 30 healthy people as controls. The 132 samples were subjected to IGRA and ELISA examinations to determine differences in MC3R levels in all groups. RESULTS: The highest mean of MC3R levels were found in the active TB group at 1.259.55 (p = 0.028) and had a positive correlation with a value of p = 0.008. In the sex group, men had the highest levels (p = 0.551). In the 30–49 year age group, the median value increased significantly in the three groups (p = 0.028), and there was a correlation between MC3R and the 17–29 year age group, although the correlation was negative (p = 0.021), in the 30–49 year age group with a positive correlation (p = 0.050). The mean MC3R value increased significantly in the overweight group in the three groups (p = 0.006) but did not significantly correlate. CONCLUSION: The high level of MC3R in TB patients is related to its role as a defence against microbes that enter the body through the immune process to prevent further infection and inflammation. Meanwhile, high levels of MC3R in excess Body mass index were associated with the function of MC3R as an inhibitor of pro-opiomelanocortin (POMC) neurons to release α-MSH.
- ItemDeteksi Gen Sulfhydryl Variable (SHV) Pada Infeksi Saluran Kemih(PENERBIT KBM INDONESIA, 2021-09-01) Nirmawati Angria
- ItemAssociation of Vitamin D Receptor Polymorphism (rs2228570, rs1544410, rs7975232, and rs731236) and Macrophage Migration Inhibitory Factor -173 G/C (rs755622) with the Susceptibility of Active Pulmonary Tuberculosis in Makassar, Indonesia(Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia, 2021-10-16) Najdah Hidayah; Irawaty Djaharuddin; Ahyar Ahmad; Rosdiana Natzir; Ilhamjaya Patellongi; Agussalim Bukhari; Irda Handayan; Andi Tenriola; Subair Subair; Handayani Halik; Muhammad Nasrum MassiBACKGROUND: The study of Vitamin D Receptor (VDR) and Macrophage Migration Inhibitory Factor (MIF) polymorphisms associated with active pulmonary tuberculosis (TB) (ATB) presents varying results. AIM: This study aimed to investigate the association between VDR rs2228570, rs1544410, rs7975232, and rs731236 and MIF -173 G/C (rs755622) single-nucleotide polymorphism (SNP), with susceptibility of developing ATB, and positivity of interferon-gamma release assay (IGRA) results (in household contact). MATERIALS AND METHODS: This study involved 83 ATB and 73 household contacts in Makassar. We checked IGRA based on ELISA in household contacts by using QuantiFERON-TB Gold Plus test, and we found that 61.64% (n = 45) of household contacts had positive IGRA. Polymorphism examination was carried out by Sanger sequencing. RESULTS: VDR rs2228570 T/T and T/C-T/T were significantly associated with a higher risk of active TB. VDR rs7975232 G/G genotype was associated with an increased risk of developing active TB compared to T/T-T/G. Haplotype analysis of VDR rs2228570, rs1544410, rs7975232, and rs731236 and combination with MIF rs755622 demonstrated that TGGTG was observed to have a higher risk of TB. CONCLUSIONS: The combination of VDR and MIF variants may contribute to the susceptibility of active tuberculosis disease.
- ItemPEMANFAATAN EKSTRAK REBUNG BAMBU BETUNG (DENDROCALAMUS ASPER) TERHADAP SEL CANCER MCF-7 SECARA IN VITRO(Poltekkes Kemenkes Bandung, 2021-10-18) Yulius Ontaha; Nur Syafah Samal; Nurfardila; Nirmawati AngriaKanker payudara merupakan tumor ganas yang tumbuh pada jaringan payudara. Penanganan penyakit kanker payudara dilakukan dengan kemoterapi, radioterapi, dan operasi. Namun, penggunaan obat-obat kemoterapi tersebut dapat menimbulkan efek samping yang berbahaya bagi tubuh seperti rambut rontok, supresi sumsum tulang, resistensi obat, disfungsi neurologi, dan toksisitas jantung. Oleh sebab itu, dibutuhkan pengobatan alternatif dengan menggunakan tanaman herbal, seperti rebung bambu betung (Dendrocalamus asper) secara ilmiah mengandung beberapa zat fitokimia dengan sifat antikanker yang tidak berbahaya bagi tubuh penderita kanker payudara. Penelitian ini bertujuan untuk mengetahui efek sitotoksik rebung bambu betung terhadap sel kanker payudara MCF-7 secara invitro dengan beberapa konsentrasi. Jenis penelitian merupakan penelitian eksperimen menggunakan sampel kanker payudara MCF7. Pengujian dilakukan dengan menggunakan metode Microtetrazolium (MTT) assay untuk mengukur efek pencegahan pertumbuhan terhadap sel kanker payudara MCF7, sehingga dapat diketahui besarnya potensi anti kanker dari ekstrak rebung bambu betung. Hasil yang diperoleh berupa tingkat efektivitas, besarnya konsentrasi, dan besarnya nilai IC50 dari ekstrak rebung bambu betung. Hasil penelitian menunjukkan bahwa ekstrak rebung bambu betung memiliki hasil rata-rata absorbansi dari konsentrasi 7,81 ppm (0.9475), 15,62 ppm (0,8772), 31,25 (0,8621), 31,25 ppm (0,8621), 62,5 ppm (0,8212), 125 ppm (0,8245), dan 250 ppm (0,8872). Hasil penelitian dapat disimpulkan bahwa semua konsentrasi ekstrak rebung bambu betung yang diuji terbukti memiliki kemampuan untuk menghambat/membunuh sel kanker payudara MCF dengan nilai IC50 1.419 µm, dikarenakan adanya kandungan Flavonoid pada Rebung bambo betung yaitu Triterpenoid yang terbukti mampu membunuh sel kanker.
- ItemAntibacterial and Anticancer Activity of Protein from Red Algae (Eucheuma cottonii)(Scholars Middle East Publishers, Dubai, United Arab Emirates, 2021-12-10) Andis Sugrani; FitrianaProtein is a major potential source of raw materials for new drugs. In this study, protein isolation of the red algae, Eucheuma cottonii was performed using a polar solvent (0.1 M Tris-HCl buffer). Purification of protein used fractionation method with ammonium sulfate, and then the last step of the isolation process performed protein dialysis. Determination of protein content was done using the Lowry method. Antibacterial activity test used agar diffusion method while for screening anticancer activity used the Brine Shrimp Lethality Test (BSLT) method. Anticancer effectiveness carried out against HeLa cancer cells. The results of antibacterial and BSLT tests on protein fractions F1 (0-20%) and F2 (20-40%) showed that these fractions were potential as anticancer agents, However, the test results of both fractions against HeLa cancer cells showed only the F2 protein fraction (20-40% fraction) was active in inhibiting the growth of HeLa cell cancer in the moderate category. The SDS PAGE electrophoresis test showed that the F2 protein fraction had a molecular weight of 26,76 kDa.
- ItemGambaran Hematologi Rutin Pada Pasien Terinfeksi Covid-19 Sebelum dan Sesudah Pemberian Terapi Plasma Konvalesen(Universitas Muhammadiyah Sidoarjo, 2021-12-31) Desyani Ariza; Andi Ulfa FerdhyantiPemeriksaan hematologi sangatlah penting dan sering diminta dibeberapa laboratorium untuk mengetahui keadaan darah dan komponen-komponennya. Tujuan penelitian ini untuk mengetahui gambaran hematologi rutin pada pasien terinfeksi covid-19 sebelum dan sesudah pemberian terapi plasma konvalesen. Rancangan penelitian ini menggunakan metode deskriptif dengan populasi sampel pasien terinfeksi Covid-19 di RS Dr. Wahidiin Sudirohusodo. Penelitian ini diikuti oleh 17 subyek yang memenuhi kriteria inklusi. Berdasarkan hasil penelitian diperoleh untuk pemeriksaan leukosit yang meningkat sebelum terapi terdapat 6 pasien dan setelah terapi terdapat 7 pasien. Pemeriksaan eritrosit yang menurun sebelum terapi terdapat 2 pasien dan setelah terapi terdapat 4 pasien. Pemeriksaan hemoglobin yang menurun sebelum terapi terdapat 3 pasien dan setelah terapi terdapat 6 pasien. Pemeriksaan hematokrit yang menurun tidak terdapat perubahan yaitu 7 pasien sebelum dan sesudah terapi. Pemeriksaan trombosit yang menurun sebelum terapi terdapat 3 pasien dan setelah terapi 4 pasien dan trombosit yang meningkat sebelum terapi terdapat 2 pasien dan setelah terapi 3 pasien. Pemeriksaan neutrofil tidak terdapat pasien yang menurun sebelum terapi dan terdapat 1 pasien yang menurun neutrofilnya setelah terapi. Untuk neutrofil yang meningkat sebelum terapi terdapat 9 pasien dan setelah terapi terdapat 7 pasien. Pemeriksaan limfosit yang menurun sebelum terapi terdapat 10 pasien dan setelah terapi terdapat 9 pasien. Kesimpulannya adalah terjadi perubahan nilai parameter hematologi rutin sebelum dan sesudah pemberian terapi plasma konvalesen pada pasien terinfeksi Covid-19.